Journal: Cell Death Discovery
Article Title: Apatinib suppresses lung cancer stem-like cells by complex interplay between β-catenin signaling and mitochondrial ROS accumulation
doi: 10.1038/s41420-021-00480-6
Figure Lengend Snippet: a Protein levels of NOX4, NQO1, SOD2, and GPX4 in A549 and H1299 cells after various concentrations of apatinib treatment. One-way ANOVA (Bonferroni’s multiple-comparison test) was used. Data are presented as mean ± SD ( n = 3). * p < 0.05, ** p < 0.01 compared to the control group. b Protein levels of NOX4, NQO1, SOD2, and GPX4 in apatinib (5 μM)-treated cells treated with/without 5 mM NAC or 200 μM H 2 O 2 for 48 h. One-way ANOVA (Bonferroni’s multiple-comparison test) was used. Data are presented as mean ± SD ( n = 3). * p < 0.05, ** p < 0.01 compared to the control group; # p < 0.05, ## p < 0.01 compared to the apatinib (5 μM) group. c Immunofluorescence analysis of JC-1 in cells after various concentrations of apatinib treatment for 24 h. Scale bar = 25 μm. One-way ANOVA (Bonferroni’s multiple-comparison test) was used. Data are presented as mean ± SD ( n = 3). ** p < 0.01 compared to the control group. d Immunofluorescence analysis of γH 2 AX (red) in apatinib (1 μM) -treated cells with/without 5 mM NAC or 200 μM H 2 O 2 . DAPI: blue. Scale bar = 100 μm. e Sphere formation assays of apatinib (1 μM)-treated with/without 5 mM NAC or 200 μM H 2 O 2 . Scale bar = 100 μm. One-way ANOVA (Bonferroni’s multiple-comparison test) was used. Data are presented as mean ± SD ( n = 3). * p < 0.05, ** p < 0.01 compared to the control group; # p < 0.05, ## p < 0.01 compared to the apatinib (1 μM) group. f Protein levels of ALDH1A1 and ABCG2 in apatinib (5 μM) treated with/without 5 mM NAC or 200 μM H 2 O 2 48 h. One-way ANOVA (Bonferroni’s multiple-comparison test) was used. Data are presented as mean ± SD ( n = 3). * p < 0.05, ** p < 0.01 compared to the control group; ## p < 0.01 compared with the apatinib (5 μM) group. g Schematic representation of the effects of apatinib on lung CSC-like properties by inducing redox imbalance.
Article Snippet: The cells or spheroids were fixed, permeabilized, incubated with 5% bovine serum albumin for 1 h, and then incubated with primary antibodies against CD133 (1:100 dilution, Proteintech), CD44 (1:200 dilution, Proteintech), β-catenin (1:200 dilution, Proteintech), ABCG2 (1:100 dilution, Cell Signaling Technology) and γH 2 AX (1:200 dilution, Cell Signaling Technology) overnight at 4 °C.
Techniques: Immunofluorescence